Protein Molecules-Stable Structure of Polypeptide Chains

Structure and folding of nascent polypeptide chains during protein translocation in the endoplasmic reticulum.

To investigate the role of protein folding and chaperone-nascent chain interactions in translocation across the endoplasmic reticulum membrane, the translocation of wild type and mutant forms of preprolactin were studied in vivo and in vitro. The preprolactin mutant studied contains an 18-amino acid substitution at the amino terminus of the mature protein, eliminating a disulfide-bonded loop do...

The polypeptide structure of immunoglobulin: experimental studies on the recombination of radiolabelled myeloma polypeptide chains.

Reconstitution of 7s Molecules from L and H Polypeptide Chains of Antibodies and Γ-globulins

Admixture of separated L and H polypeptide chains of 7S gamma-globulins under appropriate conditions has been found to result in the reconstitution of 7S molecules. The chains were mixed in 0.5 N propionic acid and when dialyzed into neutral aqueous buffers interacted to form reconstituted material in greater than 30 per cent yield. This material had sedimentation coefficients of 6S to 7S, a we...

Protein-protein Interactions among L Polypeptide Chains of Bence-jones Proteins and Human Γ-globulins

The L polypeptide chains of certain Bence-Jones proteins of group I have been found in three forms: monomers of molecular weight of about 20,000, dimers which monomerize in dissociating solvents, and dimers which are stable in such solvents. The L polypeptide chains of some Bence-Jones proteins of group II were found to occur naturally only as stable dimers. The L chains of normal human gamma-g...

Factors governing fibrillogenesis of polypeptide chains

Mai Suan Li, Nguyen Truong Co, Govardhan Reddy, C-K. Hu, J. E. Straub, and D. Thirumalai Institute of Physics, Polish Academy of Sciences, Al. Lotnikow 32/46, 02-668 Warsaw, Poland Saigon Institute for Computational Science and Technology, 6 Quarter, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam Biophysics Program, Institute for Physical Science and Technology, University of Mary...